The tests we offer can be carried out according to the UNI EN ISO 10993-5 regulation on the biological evaluation of medical
Our assays could be classified as biosafetytesting and efficacy testing. devices and they allow to highlight the safety and the efficacy of cosmetic products tested by validated in vitro methods alternative to animal testing.
- Evaluation of antioxidant activity
The ability of the cosmetic product to inhibit the formation of free radicals in cells exposed to oxidative stress, such as UVA irradiation and/or treatment with hydrogen peroxide, is analyzed, determining the anti-oxidant power. The cell viability is also assessed to highlight a potential protective activity of the sample.
- Evaluation of the protection of DNA from oxidative stress
The protective activity of a cosmetic product is evaluated by testing its potential ability to reduce the damage caused by oxidative stress to DNA using specific markers for fluorescence microscopy. A first assessment allows to detect variations of the nuclear morphology and, subsequently, the possible protection from part of the product against DNA after induction of the damage through irradiation with UV radiation.
- Evaluation of energizing
In order to check the energizing action of a cosmetic product and / or raw materials on cell cultures of human keratinocytes, the MTT assay and the analysis of the ATP content are carried out. The MTT test assesses the in vitro viability of cells exposed to different concentrations of the product compared to untreated cells, in order to highlight the maximum non-cytotoxic concentration of the product to be used in the assay. The variation of the ATP content is evaluated in order to verify whether the product and / or raw material possesses an energizing activity.
- Evaluation of regenerating activity
In order to assess the regenerative action of a cosmetic product on cell cultures of human keratinocytes, MTT test is carried out, which allows to evaluate the viability of cells in vitro exposed to different concentrations of product and to highlight, if it exists, the concentration can stimulate cell growth. The protein synthesis is also evaluated in order to verify if the product/raw material has a regenerating activity.
- Evaluation of anti-inflammatory activity
Inflammatory reactions generate pathologies by contact and they are a very important field of study to understand the effects of cosmetics on human skin and appendages. Tests involve the identification of new markers intra/extracellular and quantification of cytokines released in the cells in contact with the cosmetic/ devices to analyze.
- Evaluation of pro-sensitizing potential
This test is able to evaluate the potential pro-sensitizing caused by raw materials/finished products for medical and cosmetic use by flow cytometric analysis of membrane antigens. Through the analysis of the expression of co-stimulatory molecules, it is evaluated the immunological reactivity of immune cells (monocytes) exposed to contact with the substance under examination, after having identified the non-toxic concentrations using MTT test.
- Evaluation of DNA damage
Through the technique of the Comet is possible to study the direct DNA damage induced by UV radiation and the possible protective capacity of the sample. The damage that can be identified are single-strand breaks, in case you decide to follow the standard procedure; otherwise it is possible to highlight the oxidation of the bases of DNA through the introduction of specific enzymes capable of recognizing oxidized bases distinguishing between purines and pyrimidines oxidized.
- Evaluation of moisturizing activity
One of the main effects of photoaging induced by ultraviolet radiation is the dehydration of the skin. To investigate through alternative methods in vitro study of this activity, it is possible to evaluate the expression of proteins capable of forming channels in the cell membrane in order to facilitate the transport of water, such as aquaporins, using western blotting technique. The functionality of these proteins influences the motility of keratinocytes, studied by the wound healing technique.
- Skin irritation evaluation on a 3D epidermis model
The assay evaluates the potential irritant effect caused by the cosmetic product / medical device on an in vitro three-dimensional human skin model, according to the standard ISO 10993-10: 2010 reference.
- Mucoadhesion test
The mucous membrane contact with harmful substances or irritants may result in its penetration, causing severe degenerative effects. For this reason, the administration of substances with mucoadhesive properties, may contribute to protect the mucosa from the risk of aggression.
In order to assess the mucoadhesion of products to be used for the treatment of mucous membranes, an assessment of the binding inhibition of specific proteins is performed.
- Antiplaque activity test
The evaluation of antiplaque activity of a product is based on the ability of the product itself to inhibit the growth of the main bacteria responsible for dental plaque.
- In vitro evaluation of the barrier effect
The assay evaluates the possible barrier effect produced by the cosmetic product / medical device after the damage induced by an irritating agent on a human epithelial three-dimensional model, according to validated protocols.